Anticorps Recombinant de lapin anti-WDR21C

• Phare
• Validé par KD/KO

WDR21C Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240402H3

N° de cat : 83802-4-RR

Synonymes

240402H3, DCAF4L2, DDB1- and CUL4-associated factor 4-like protein 2, WD repeat domain 21C, WD repeat-containing protein 21C

---

Galerie de données de validation

• 

  WB analysis using 83802-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83802-4-RR (WDR21C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 83802-4-RR

  WB result of WDR21C antibody (83802-4-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR21C transfected HEK-293 cells.

• 

  Affinity and Kinetic Characterization of 83802-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83802-4-RR against Human WDR21C were performed. The affinity constant is 0.479 nM.

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules U-251

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83802-4-RR cible WDR21C dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: WDR21C Protéine recombinante Ag30748
• Nom complet: WD repeat domain 21C
• Masse moléculaire calculée: 395 aa, 44 kDa
• Poids moléculaire observé: 44-50 kDa
• Numéro d’acquisition GenBank: BC111778
• Symbole du gène: WDR21C
• Identification du gène (NCBI): 138009
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WDR21C (WD repeat-containing protein 21C), also known as DCAF4L2. It is expected to be located in the cytoplasm, which is enriched in testis. WDR21C is a member of WD-repeat proteins, which commonly serve as mediators of protein-protein interplay. It is reported that elevated WDR21C expression in human colorectal cancer (CRC) significantly correlated with a more advanced clinical stage as in lymphatic and distant metastasis. More importantly, elevated WDR21C expression is an independent prognosis factor for survival (PMID: 27158335). The molecular weight of WDR21C is 43 kDa.

{{ptg:RelatedPrimaryAntibodies}}