Anticorps Recombinant de lapin anti-WDR24

• Phare
• Validé par KD/KO

WDR24 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251220G1

N° de cat : 86464-1-PBS

Synonymes

C16orf21, EC:2.3.2.27, GATOR2 complex protein WDR24, JFP7, WD repeat-containing protein 24

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Galerie de données de validation

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  WB analysis using 86464-1-RR (same clone as 86464-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86464-1-RR (WDR24 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86464-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 86464-1-RR (same clone as 86464-1-PBS)

  WB result of WDR24 antibody (86464-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR24 transfected HEK-293 cells. This data was developed using the same antibody clone with 86464-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 86464-1-RR (same clone as 86464-1-PBS)

  IP result of anti-WDR24 (IP:86464-1-RR, 4ug; Detection:86464-1-RR 1:2000) with HEK-293 cells lysate 1200 ug. This data was developed using the same antibody clone with 86464-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86464-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86464-1-RR against Human WDR24 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86464-1-PBS cible WDR24 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: WDR24 Protéine recombinante Ag14774
• Nom complet: WD repeat domain 24
• Masse moléculaire calculée: 920 aa, 102 kDa
• Poids moléculaire observé: 88 kDa
• Numéro d’acquisition GenBank: BC008025
• Symbole du gène: WDR24
• Identification du gène (NCBI): 84219
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

WDR24 is a component of the GATOR2 complex which has five known subunits (WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway. (PMID: 23723238; 25263562)

{{ptg:RelatedPrimaryAntibodies}}