Anticorps Polyclonal de lapin anti-WDR32

WDR32 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20483-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 20483-1-AP (same clone as 20483-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20483-1-AP (WDR32 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20483-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 20483-1-AP (same clone as 20483-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20483-1-AP (WDR32 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20483-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 20483-1-AP (same clone as 20483-1-PBS)

  IP result of anti-WDR32 (IP:20483-1-AP, 4ug; Detection:20483-1-AP 1:300) with HepG2 cells lysate 6000ug. This data was developed using the same antibody clone with 20483-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 20483-1-AP (same clone as 20483-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20483-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 20483-1-AP (same clone as 20483-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20483-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 20483-1-AP (same clone as 20483-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20483-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20483-1-AP (same clone as 20483-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20483-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 20483-1-AP (same clone as 20483-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 20483-1-AP (WDR32 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20483-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20483-1-PBS cible WDR32 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WDR32 Protéine recombinante Ag14360
• Nom complet: WD repeat domain 32
• Masse moléculaire calculée: 559 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC003520
• Symbole du gène: WDR32
• Identification du gène (NCBI): 79269
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

WDR32, containing seven WD repeats, is also termed DDB1- and CUL4-associated factor 10 (DCAF10), which may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex (PMID: 16949367). WDR32 is able to interact with DDB1 (PMID: 16949367). WDR32 is supposed to be implicated in process of ubiquitin-dependent protein degradation system. Two isoforms are described and produced by alternative splicing. Proteintech's WDR32 antibody 20483-1-AP majorly detects WDR32 isoform1 with a theoretical MW of 61 kDa.

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