Anticorps Polyclonal de lapin anti-WDR43

WDR43 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30227-1-PBS

Synonymes

KIAA0007, WD repeat domain 43

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Galerie de données de validation

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  WB analysis using 30227-1-AP (same clone as 30227-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30227-1-AP (WDR43 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 30227-1-AP (same clone as 30227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 30227-1-AP (same clone as 30227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 30227-1-AP (same clone as 30227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat ovary using 30227-1-AP (same clone as 30227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30227-1-PBS cible WDR43 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WDR43 Protéine recombinante Ag30585
• Nom complet: WD repeat domain 43
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: NM_015131
• Symbole du gène: WDR43
• Identification du gène (NCBI): 23160
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

WDR43, a WD40 domain-containing protein, is a conserved component of the small-subunit processome (SSUP), which mediates pre-18S ribosomal RNA (rRNA) transcription and processing in the nucleolus and is critical for ribosome biogenesis in yeast. Its mutation in zebrafish causes a variety of early developmental defects in specific tissues. WDR43 directly promotes ESC self-renewal by maintaining high-level expression of its target genes involved in pluripotency programs. (PMID: 28880863, 24497835, 31128943)

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