Anticorps Polyclonal de lapin anti-WDR45/WIPI4

• Phare
• Validé par KD/KO

WDR45/WIPI4 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 19194-1-AP

Synonymes

JM5, WD repeat domain 45, WDR45, WDRX1, WDRXI4, WIPI 4, WIPI4

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 19194-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19194-1-AP (WDR45 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19194-1-AP (WDR45 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 19194-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19194-1-AP (WDR45 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 19194-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19194-1-AP (WDR45 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 19194-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 19194-1-AP (WDR45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 19194-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19194-1-AP (WDR45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu de muscle squelettique de rat
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IP: See 1 publications below

Informations sur le produit

19194-1-AP cible WDR45/WIPI4 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WDR45/WIPI4 Protéine recombinante Ag6741
• Nom complet: WD repeat domain 45
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC000464
• Symbole du gène: WDR45
• Identification du gène (NCBI): 11152
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WD repeat domain phosphoinositide-interacting protein 4 (WDR45) is also named as WDRX1, WDRXI4 and WIPI4, and belongs to the WD repeat PROPPIN family. WDR45 is highly conserved in mammals, and the amino acid sequences of the human, mouse, pig, bovine and horse protein have more than 97% identity (PMID:20505359). WDR45 is component of the autophagy machinery that controls the major intracellular degradation process (PMID:23435086, PMID:28561066). Together with WIPI1, WIPI2 and WIPI3/WDR45B, it forms the WIPI (WD repeat domain, phosphoinositide interacting) protein family. And this is why WDR45 is also known as WIPI4 (PMID:11814058). The best characterized molecular function of WDR45 is promoting lipid transfer together with ATG2 proteins between adjacent membranes. This function at the phagophore-ER membrane contact sites is important for autophagy (PMID:30185561, PMID:31721365). WDR45 is activated by the STK11/AMPK signaling pathway upon starvation. WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes (PMID:28561066).

Publications

Species	Application	Title
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
mouse	WB,IP	Sci Adv; Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolism.; Authors - Alexander S Häusl ; KD Validated
human	WB	Int J Mol Sci; Iron Accumulation and Changes in Cellular Organelles in WDR45 Mutant Fibroblasts.; Authors - Hye Eun Lee ; KD Validated
human	WB	J Neurochem; A neurodegeneration gene, WDR45, links impaired ferritinophagy to iron accumulation; Authors - Luisa Aring ; KO Validated
human	WB	Am J Med Genet A; Autophagic defects observed in fibroblasts from a patient with β-propeller protein-associated neurodegeneration.; Authors - Jae-Hyeok Lee
human	WB	Eur J Med Genet; Early onset developmental delay and epilepsy in pediatric patients with WDR45 variants.; Authors - Hongbo Chen