Anticorps Polyclonal de lapin anti-WDR45/WIPI4

• Phare
• Validé par KD/KO

WDR45/WIPI4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 19194-1-PBS

Synonymes

WDR45, WDRX1, WD repeat-containing protein 45, WD repeat domain phosphoinositide-interacting protein 4, WD repeat domain 45

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 19194-1-AP (same clone as 19194-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19194-1-AP (WDR45 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

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  WB analysis using 19194-1-AP (same clone as 19194-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19194-1-AP (WDR45 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 19194-1-AP (same clone as 19194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19194-1-AP (WDR45 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 19194-1-AP (same clone as 19194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19194-1-AP (WDR45 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19194-1-AP (same clone as 19194-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WDR45/WIPI4 antibody (19194-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19194-1-AP (same clone as 19194-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19194-1-AP (WDR45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19194-1-PBS cible WDR45/WIPI4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WDR45/WIPI4 Protéine recombinante Ag6741
• Nom complet: WD repeat domain 45
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC000464
• Symbole du gène: WDR45
• Identification du gène (NCBI): 11152
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

WD repeat domain phosphoinositide-interacting protein 4 (WDR45) is also named as WDRX1, WDRXI4 and WIPI4, and belongs to the WD repeat PROPPIN family. WDR45 is highly conserved in mammals, and the amino acid sequences of the human, mouse, pig, bovine and horse protein have more than 97% identity (PMID:20505359). WDR45 is component of the autophagy machinery that controls the major intracellular degradation process (PMID:23435086, PMID:28561066). Together with WIPI1, WIPI2 and WIPI3/WDR45B, it forms the WIPI (WD repeat domain, phosphoinositide interacting) protein family. And this is why WDR45 is also known as WIPI4 (PMID:11814058). The best characterized molecular function of WDR45 is promoting lipid transfer together with ATG2 proteins between adjacent membranes. This function at the phagophore-ER membrane contact sites is important for autophagy (PMID:30185561, PMID:31721365). WDR45 is activated by the STK11/AMPK signaling pathway upon starvation. WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes (PMID:28561066).

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