Anticorps Polyclonal de lapin anti-WDR74

WDR74 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 20631-1-AP

Synonymes

NOP seven associated protein 1, NOP seven-associated protein 1, NSA1, WD repeat domain 74, WD repeat-containing protein 74

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Galerie de données de validation

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  WB analysis using 20631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20631-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20631-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 20631-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules K-562, cellules L02, cellules MCF-7, cellules MDA-MB-231

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

20631-1-AP cible WDR74 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WDR74 Protéine recombinante Ag14617
• Nom complet: WD repeat domain 74
• Masse moléculaire calculée: 385 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC006351
• Symbole du gène: WDR74
• Identification du gène (NCBI): 54663
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WDR74, also known as WD repeat domain 74, is a protein-coding gene that plays important roles in various biological processes. WDR74 is involved in rRNA processing and ribosomal large subunit biogenesis. It is a 60S ribosome assembly factor, which is irreplaceable in ribosome biogenesis and is closely related to protein synthesis. WDR74 can induce nuclear β-catenin accumulation and activate Wnt-responsive genes, thus promoting cell proliferation and metastasis in various cancers.

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