Anticorps Recombinant de lapin anti-WDR74

WDR74 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251046A3

N° de cat : 86361-2-PBS

Synonymes

NSA1, WD repeat domain 74, WD repeat-containing protein 74

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Galerie de données de validation

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  WB analysis using 86361-2-RR (same clone as 86361-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86361-2-RR (WDR74 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86361-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86361-2-RR (same clone as 86361-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WDR74 antibody (86361-2-RR, Clone: 251046A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86361-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86361-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86361-2-RR against Human WDR74 were performed. The affinity constant is 1.02 nM.

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Informations sur le produit

86361-2-PBS cible WDR74 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: WDR74 Protéine recombinante Ag14617
• Nom complet: WD repeat domain 74
• Masse moléculaire calculée: 385 aa, 42 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC006351
• Symbole du gène: WDR74
• Identification du gène (NCBI): 54663
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

WDR74, also known as WD repeat domain 74, is a protein-coding gene that plays important roles in various biological processes. WDR74 is involved in rRNA processing and ribosomal large subunit biogenesis. It is a 60S ribosome assembly factor, which is irreplaceable in ribosome biogenesis and is closely related to protein synthesis. WDR74 can induce nuclear β-catenin accumulation and activate Wnt-responsive genes, thus promoting cell proliferation and metastasis in various cancers.

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