Anticorps Polyclonal de lapin anti-WDR82

WDR82 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 21354-1-AP

Synonymes

MST107, MSTP107, PRO2730, PRO34047, SWD2, TMEM113, WD repeat domain 82, WDR82, WDR82A

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Galerie de données de validation

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  WB analysis of HL-60 using 21354-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21354-1-AP (WDR82 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 21354-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21354-1-AP (WDR82 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21354-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21354-1-AP (WDR82 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21354-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21354-1-AP (WDR82 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HL-60, Jurkat cells; K-562 cells
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

21354-1-AP cible WDR82 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WDR82 Protéine recombinante Ag15762
• Nom complet: WD repeat domain 82
• Masse moléculaire calculée: 313 aa, 35 kDa
• Poids moléculaire observé: 33-35 kDa
• Numéro d’acquisition GenBank: BC018941
• Symbole du gène: WDR82
• Identification du gène (NCBI): 80335
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Sci Adv; EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency; Authors - Chikako Okubo
human	WB	J Exp Clin Cancer Res; Radiotherapy resistance driven by Asparagine endopeptidase through ATR pathway modulation in breast cancer; Authors - Macarena Morillo-Huesca

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mia (Verified Customer) (07-01-2025)

This antibody was incredibly helpful for my summer project while in medical school. It was used at a 1:100 dilution for staining BHPrS1 cells, and we are planning to drop the dilution to 1:500. It was also used at a 1:200 dilution for IHC of mouse and human prostate tissue, and worked wonderfully.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:200 for IHC, 1:100 for ICC
• Cell Tissue Type: Mouse & human prostate, BHPrS1 cells

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