Anticorps Polyclonal de lapin anti-WEE1

WEE1 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29474-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 29474-1-AP (same clone as 29474-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29474-1-AP (WEE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29474-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29474-1-PBS cible WEE1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WEE1 Protéine recombinante Ag30194
• Nom complet: WEE1 homolog (S. pombe)
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 72 kDa,110 kDa
• Numéro d’acquisition GenBank: BC051831
• Symbole du gène: WEE1
• Identification du gène (NCBI): 7465
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

WEE1 kinase is a serine-threonine kinase that regulates G2/M checkpoint transition. WEE1 triggers G2/M arrest through inhibitory phosphorylation on Tyr15 of CDK1 (Cdc2) and preventing entry into mitosis to allow DNA repair during DNA damage (PMID: 27427153).The predicted native molecular weight for Wee1 based on the intact human Wee1 cDNA sequence is 72 kDa, whereas the 95-110 kDa protein is a highly modified product (PMID: 7568188). Others have shown that Wee1 is 70-72 kDa and 49-55 kDa (PMID: 12214061).

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