Anticorps Polyclonal de lapin anti-WFS1

• Phare
• Validé par KD/KO

WFS1 Polyclonal Antibody for IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26995-1-PBS

Synonymes

WFRS, WFS, WOLFRAMIN

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Galerie de données de validation

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  IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:400 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:800 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 26995-1-AP (same clone as 26995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26995-1-PBS cible WFS1 dans les applications de IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WFS1 Protéine recombinante Ag25724
• Nom complet: Wolfram syndrome 1 (wolframin)
• Masse moléculaire calculée: 890 aa, 100 kDa
• Numéro d’acquisition GenBank: BC030130
• Symbole du gène: WFS1
• Identification du gène (NCBI): 7466
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss. Wolframin assembles into higher molecular weight complexes of approximately 400 kDa in the membrane(PMID: 12913071)

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