Anticorps Recombinant de lapin anti-NSD3

• Phare
• Validé par KD/KO

NSD3 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230499C2

N° de cat : 83116-2-RR

Synonymes

WHSC1L1, DC28, EC:2.1.1.370, Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3

---

Galerie de données de validation

• 

  WB analysis of A549 using 83116-2-RR

  WB result of NSD3 antibody (83116-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSD3 transfected A549 cells.

• 

  WB analysis using 83116-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83116-2-RR (WHSC1L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung squamous cell carcinoma using 83116-2-RR

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 83116-2-RR (NSD3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 83116-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NSD3 antibody (83116-2-RR, Clone: 230499C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 83116-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug NSD3 Recombinant antibody (83116-2-RR, Clone:230499C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83116-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83116-2-RR against Human NSD3 were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules K-562
• Résultats positifs en IHC: human lung squamous cell carcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83116-2-RR cible NSD3 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NSD3 Protéine recombinante Ag1894
• Nom complet: Wolf-Hirschhorn syndrome candidate 1-like 1
• Masse moléculaire calculée: 1437 aa, 162 kDa
• Poids moléculaire observé: 160-170 kDa, 68-72 kDa
• Numéro d’acquisition GenBank: BC012059
• Symbole du gène: WHSC1L1
• Identification du gène (NCBI): 54904
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).

{{ptg:RelatedPrimaryAntibodies}}