Anticorps Recombinant de lapin anti-WIBG

WIBG Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242800G10

N° de cat : 86064-1-PBS

Synonymes

Partner of Y14 and mago, Protein wibg homolog, PYM, PYM homolog 1 exon junction complex-associated factor, PYM1

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Galerie de données de validation

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  WB analysis using 86064-1-RR (same clone as 86064-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86064-1-RR (WIBG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86064-1-PBS in a different storage buffer formulation.

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  WB analysis using 86064-1-RR (same clone as 86064-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86064-1-RR (WIBG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86064-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 86064-1-RR (same clone as 86064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 86064-1-RR (WIBG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86064-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 86064-1-RR (same clone as 86064-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using WIBG antibody (86064-1-RR, Clone: 242800G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86064-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02733-1

  The mean WIBG concentration was determined to be 112.9 ng/mL in U2OS cell extract based on a 1.2 mg/mL extract load, 201.7 ng/mL in A549 cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02733-1

  Cytometric bead array standard curve of MP02733-1, WIBG Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86064-1-PBS. Detection antibody: 86064-2-PBS. Standard: Ag6276. Range: 1.562-200 ng/mL.

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Informations sur le produit

86064-1-PBS cible WIBG dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: WIBG Protéine recombinante Ag6276
• Nom complet: within bgcn homolog (Drosophila)
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23-27 kDa
• Numéro d’acquisition GenBank: BC006135
• Symbole du gène: WIBG
• Identification du gène (NCBI): 84305
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Protein WIBG homolog, also known as PYM1 (Partner of Y14 and mago), is a cytoplasmic RNA-binding protein, that can interact with the ribosome and EJC, may act as an EJC destabilizing factor, preventing EJC accumulation at positions away from exon-exon junctions (non-canonical position).

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