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Anticorps Recombinant de lapin anti-WNK4

WNK4 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250156A10

N° de cat : 85886-4-PBS

Synonymes

EC:2.7.11.1, PHA2B, PRKWNK4, Protein kinase lysine-deficient 4, Serine/threonine-protein kinase WNK4

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of HepG2 cells using WNK4 Recombinant antibody (85886-4-RR)

    WB analysis of HepG2 using 85886-4-RR (same clone as 85886-4-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 85886-4-RR (WNK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85886-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using WNK4 Recombinant antibody (85886-4-RR)

    WB analysis using 85886-4-RR (same clone as 85886-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85886-4-RR (WNK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85886-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85886-4-RR

    Affinity and Kinetic Characterization of 85886-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85886-4-RR against Human WNK4 were performed. The affinity constant is 0.926 nM.

Informations sur le produit

85886-4-PBS cible WNK4 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène WNK4 Protéine recombinante Ag17773
Nom complet WNK lysine deficient protein kinase 4
Masse moléculaire calculée 1243 aa, 135 kDa
Poids moléculaire observé 220 kDa
Numéro d’acquisition GenBankBC136664
Symbole du gène WNK4
Identification du gène (NCBI) 65266
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

WNK4 (With No Lysine Kinase 4) is a member of the WNK family of serine/threonine protein kinases. It plays a crucial role in regulating electrolyte balance and blood pressure by modulating the activity of ion transporters in the kidney. WNK4 is primarily involved in the regulation of sodium and potassium homeostasis. It achieves this by phosphorylating and activating the kinases SPAK and OSR1, which in turn regulate the activity of the sodium-chloride cotransporter (NCC) in the distal convoluted tubule of the kidney. Additionally, by promoting sodium reabsorption and potassium secretion, WNK4 contributes to the regulation of blood pressure. Increased activity of WNK4 can lead to hypertension. (PMID: 24655003; 33491560)

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