Anticorps Recombinant de lapin anti-WNK4

WNK4 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250156A10

N° de cat : 85886-4-RR

Synonymes

EC:2.7.11.1, PHA2B, PRKWNK4, Protein kinase lysine-deficient 4, Serine/threonine-protein kinase WNK4

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Galerie de données de validation

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  WB analysis of HepG2 using 85886-4-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 85886-4-RR (WNK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85886-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85886-4-RR (WNK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85886-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85886-4-RR against Human WNK4 were performed. The affinity constant is 0.926 nM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A375

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85886-4-RR cible WNK4 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: WNK4 Protéine recombinante Ag17773
• Nom complet: WNK lysine deficient protein kinase 4
• Masse moléculaire calculée: 1243 aa, 135 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC136664
• Symbole du gène: WNK4
• Identification du gène (NCBI): 65266
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WNK4 (With No Lysine Kinase 4) is a member of the WNK family of serine/threonine protein kinases. It plays a crucial role in regulating electrolyte balance and blood pressure by modulating the activity of ion transporters in the kidney. WNK4 is primarily involved in the regulation of sodium and potassium homeostasis. It achieves this by phosphorylating and activating the kinases SPAK and OSR1, which in turn regulate the activity of the sodium-chloride cotransporter (NCC) in the distal convoluted tubule of the kidney. Additionally, by promoting sodium reabsorption and potassium secretion, WNK4 contributes to the regulation of blood pressure. Increased activity of WNK4 can lead to hypertension. (PMID: 24655003; 33491560)

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