Anticorps Polyclonal de lapin anti-WNT2

• Phare
• Validé par KD/KO

WNT2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 27214-1-AP

Synonymes

Int 1 like protein 1, Int 1 related protein, INT1L1, IRP, Protein Wnt 2, WNT2

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Galerie de données de validation

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  WB analysis using 27214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27214-1-AP (WNT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27214-1-AP (WNT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27214-1-AP (WNT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 27214-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27214-1-AP (WNT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27214-1-AP (WNT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 27214-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using WNT2 antibody (27214-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Jurkat using 27214-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human WNT2 (27214-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules MCF-7, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 1 publications below

Informations sur le produit

27214-1-AP cible WNT2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WNT2 Protéine recombinante Ag25845
• Nom complet: wingless-type MMTV integration site family member 2
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40 kDa, 34 kDa
• Numéro d’acquisition GenBank: BC029854
• Symbole du gène: WNT2
• Identification du gène (NCBI): 7472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.

Publications

Species	Application	Title
mouse	WB	Clin Transl Med; Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.; Authors - Xi Dong
human	WB	Aging (Albany NY); LncRNA-HAGLR motivates triple negative breast cancer progression by regulation of WNT2 via sponging miR-335-3p.; Authors - Liting Jin
mouse	WB	FASEB J; Bisphenol A exposure alters placentation and causes preeclampsia-like features in pregnant mice involved in reprogramming of DNA methylation of WNT2.; Authors - Yunzhen Ye
human	WB	Phytother Res; Capsaicin suppressed activity of prostate cancer stem cells by inhibition of Wnt/β-catenin pathway.; Authors - Mingming Zhu
mouse	WB	Brain Res Bull; Excess folic acid supplementation before and during pregnancy and lactation activates β-catenin in the brain of male mouse offspring.; Authors - Qian Wu
human	WB	Cancer Med; Mycoplasma hyorhinis infection promotes gastric cancer cell motility via β-catenin signaling.; Authors - Xia Liu ; KD Validated