Anticorps Polyclonal de lapin anti-WNT3A

WNT3A Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(44)

N° de cat : 26744-1-AP

Synonymes

Protein Wnt 3a, Protein Wnt-3a

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Galerie de données de validation

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  WB analysis of fetal human brain using 26744-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 26744-1-AP (WNT3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 26744-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26744-1-AP (WNT3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 43 publications below
• IF: See 1 publications below

Informations sur le produit

26744-1-AP cible WNT3A dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WNT3A Protéine recombinante Ag25051
• Nom complet: wingless-type MMTV integration site family, member 3A
• Masse moléculaire calculée: 352 aa, 39 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC103921
• Symbole du gène: WNT3A
• Identification du gène (NCBI): 89780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WNT3A belongs to wingless-type MMTV integration site family, and abnormal Wnt signalling is often associated with severe human diseases, including cancer, osteoporosis and other degenerative disorders. WNT3A is a secreted glycoprotein that is involved in both canonical and non-canonical Wnt signaling pathways. The canonical Wnt pathway, also known as the β-catenin-dependent pathway, regulates gene expression by stabilizing β-catenin, which then translocates to the nucleus and interacts with transcription factors like TCF/LEF. It's shown to promote trophectoderm formation in embryonic stem cells. It's reported that the canonical Wnt/β-catenin signaling pathway also contributes to the carcinogenesis and progression of lung cancer cell lines. The non-canonical pathways, which are β-catenin-independent, are involved in cytoskeletal organization and cell polarity.

Publications

Species	Application	Title
mouse	WB	Food Funct; Procyanidin B1 and p-coumaric acid from whole highland barley ameliorated HFD-induced impaired glucose tolerance via small intestinal barrier and hepatic glucose metabolism; Authors - Zehua Liu
rat	WB	Aging (Albany NY); Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats.; Authors - Xin Zhang
rat	WB	Arch Toxicol; Developmental exposure to nonylphenol induced rat axonal injury in vivo and in vitro.; Authors - Siyao Li
human	WB	Genes Nutr; Arjunolic acid inhibits Wnt3a-mediated macrophage M2 polarization to suppress osteosarcoma progression; Authors - Jun Li
human	WB	Apoptosis; SKI knockdown suppresses apoptosis and extracellular matrix degradation of nucleus pulposus cells via inhibition of the Wnt/β-catenin pathway and ameliorates disc degeneration.; Authors - Zuo-Long Wu
human	WB	Biomed Pharmacother; BAIAP2L2 promotes the progression of gastric cancer via AKT/mTOR and Wnt3a/β-catenin signaling pathways.; Authors - Jianing Liu

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