Anticorps Polyclonal de lapin anti-WNT5A/B

WNT5A/B Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 29793-1-AP

Synonymes

WNT5A, hWNT5A, Protein Wnt 5a, Protein Wnt-5a, WNT5A B

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Galerie de données de validation

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  WB analysis using 29793-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29793-1-AP (WNT5A/B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 29793-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29793-1-AP (WNT5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 29793-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT5A antibody (29793-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HeLa, cellules MCF-7, cellules SKOV-3
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

29793-1-AP cible WNT5A/B dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WNT5A/B Protéine recombinante Ag31507
• Nom complet: wingless-type MMTV integration site family, member 5A
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC064694
• Symbole du gène: WNT5A
• Identification du gène (NCBI): 7474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptides.

Publications

Species	Application	Title
rat	WB	J Biol Chem; Exosomal miR-23a-3p derived from human umbilical cord mesenchymal stem cells promotes remyelination in central nervous system demyelinating diseases by targeting Tbr1/Wnt pathway; Authors - Danqing Qin
human	WB,IHC	J Orthop Res; Increased Wnt5a/ROR2 signaling is associated with chondrogenesis in meniscal degeneration; Authors - Yusuke Inoue
rat	WB	Biochim Biophys Acta Mol Cell Res; Wnt/β-catenin signaling inhibits oxidative stress-induced ferroptosis to improve interstitial cystitis/bladder pain syndrome by reducing NF-κB; Authors - Weilin Fang
human	WB,IF	Noncoding RNA Res; LncRNA NEAT1-206 regulates autophagy of human umbilical cord mesenchymal stem cells through the WNT5A/Ca2+ signaling pathway under senescence stress; Authors - Weili Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (12-22-2023)

Worked well for WB at 1:2000 in ADSCs

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: ADSCs

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