Anticorps Polyclonal de lapin anti-WNT7A/B

WNT7A/B Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 10605-1-AP

Synonymes

Protein Wnt 7a, WNT7A, WNT7A/B, WNT7B

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Galerie de données de validation

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  WB analysis using 10605-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10605-1-AP (WNT7A/B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 10605-1-AP

  IP Result of anti-WNT7A (IP:10605-1-AP, 4ug; Detection:10605-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 10605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10605-1-AP (WNT7A antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 10605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10605-1-AP (WNT7A antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 10605-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10605-1-AP (WNT7A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 10605-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug WNT7A antibody (10605-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu hépatique de souris
• Résultats positifs en IP: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

10605-1-AP cible WNT7A/B dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WNT7A/B Protéine recombinante Ag0874
• Nom complet: wingless-type MMTV integration site family, member 7A
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 35-39 kDa
• Numéro d’acquisition GenBank: BC008811
• Symbole du gène: WNT7A
• Identification du gène (NCBI): 7476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Wnt gene family encodes secreted signaling molecules that bind to frizzled receptors and influence oncogenesis and developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt7a is normally expressed in several organs, including the lung, testis, lymph node, and brain. As an oncogenic autocrine glycoprotein, Wnt7a promotes tumor invasion and distant metastasis with cancer-associated fibroblasts. Wnt7b is required for endothelial cells derived from pluripotent stem cells to acquire blood-brain barrier properties and may play an important role in the development of myopia in humans. This antibody can both recognize WNT7A and WNT7B.

Publications

Species	Application	Title
human	WB, IHC	Front Oncol; The Role of miR-640: A Potential Suppressor in Breast Cancer via Wnt7b/β-catenin Signaling Pathway.; Authors - Chun Tang
human	WB,IHC	Oncol Rep; Wnt7a predicts poor prognosis, and contributes to growth and metastasis in tongue squamous cell carcinoma.; Authors - Bo Jia ; KD Validated
mouse	WB	Behav Brain Res; OGDHL ameliorates cognitive impairment and Alzheimer's disease-like pathology via activating Wnt/β-catenin signaling in Alzheimer's disease mice.; Authors - Li Yao
	WB	Oncol Rep; RNA-binding motif protein 5 negatively regulates the activity of Wnt/β-catenin signaling in cigarette smoke-induced alveolar epithelial injury.; Authors - Yu-Qiu Hao
mouse	WB,IHC	Neurol Res; Wnt signaling pathway is involved in the pathogenesis of amyotrophic lateral sclerosis in adult transgenic mice.; Authors - Chen Yanchun Y
mouse	WB	NAR Cancer; EVI1 activates tumor-promoting transcriptional enhancers in pancreatic cancer.; Authors - Hwa-Ryeon Kim