Anticorps Polyclonal de lapin anti-WNT7A

WNT7A Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27177-1-PBS

Synonymes

Protein Wnt-7a, Protein Wnt 7a

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Galerie de données de validation

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  WB analysis using 27177-1-AP (same clone as 27177-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27177-1-AP (WNT7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27177-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 27177-1-AP (same clone as 27177-1-PBS)

  IP result of anti-WNT7A (IP:27177-1-AP, 4ug; Detection:27177-1-AP 1:2000) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 27177-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 27177-1-AP (same clone as 27177-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WNT7A antibody (27177-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 27177-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27177-1-PBS cible WNT7A dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WNT7A Protéine recombinante Ag26060
• Nom complet: wingless-type MMTV integration site family, member 7A
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 30-39 kDa
• Numéro d’acquisition GenBank: BC008811
• Symbole du gène: WNT7A
• Identification du gène (NCBI): 7476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

WNT7A belongs to the Wnt family. WNT7A plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PMID: 16826533). WNT7A is required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PMID:30026314). WNT7A is highly expressed in basal cells and is secreted in abundance by basal cells (PMID: 36318668).

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