Anticorps Polyclonal de lapin anti-WRAP53

• Phare
• Validé par KD/KO

WRAP53 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 14761-1-AP

Synonymes

WDR79, TCAB1, WD repeat-containing protein 79, WD40 repeat-containing protein antisense to TP53 gene, WRAP53beta

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Galerie de données de validation

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  WB analysis using 14761-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14761-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14761-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14761-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14761-1-AP

  IP result of anti-WRAP53 (IP:14761-1-AP, 4ug; Detection:14761-1-AP 1:300) with HeLa cells lysate 2480ug.

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  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HeLa, tissu rénal humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire de souris, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

14761-1-AP cible WRAP53 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WRAP53 Protéine recombinante Ag6522
• Nom complet: WD repeat containing, antisense to TP53
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 72-75 kDa
• Numéro d’acquisition GenBank: BC002336
• Symbole du gène: WRAP53
• Identification du gène (NCBI): 55135
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WRAP53 (WD40-encoding RNA Antisense to p53), also known as TCAB1 and WDR79, is a multifunctional protein encoded by a gene that produces both a coding mRNA and a regulatory antisense RNA targeting the tumor suppressor p53. Initially identified for its role in maintaining genomic stability by regulating p53 expression, WRAP53 is crucial for telomerase assembly and localization to Cajal bodies, impacting telomere maintenance. It also participates in DNA damage repair and cellular stress responses. The calculated molecular weight of WRAP53 is about 59 kDa, but the actual observed molecular weight is about 72-75 kDa, which may be due to modification.

Publications

Species	Application	Title
human	IF	Genes Dev; The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair.; Authors - Sofia Henriksson
human	WB	Nat Chem Biol; Observation of processive telomerase catalysis using high-resolution optical tweezers.; Authors - Eric M Patrick
human	WB	Oncogenesis; RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks.; Authors - Qunsong Tan ; KD Validated
	WB	Aging (Albany NY); Relationship between senescence in macaques and bone marrow mesenchymal stem cells and the molecular mechanism.; Authors - Xing-Hua Pan
human	WB,IF	RNA Biol; Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response.; Authors - Christos Coucoravas ; KD Validated
human	WB	Cancer Cell Int; Suppression of TCAB1 expression induced cellular senescence by lessening proteasomal degradation of p21 in cancer cells.; Authors - Jing Niu

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