Anticorps Recombinant de lapin anti-WRAP53

WRAP53 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251391B8

N° de cat : 86487-1-RR

Synonymes

WDR79, TCAB1, WD repeat-containing protein 79, WD40 repeat-containing protein antisense to TP53 gene, WRAP53beta

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Galerie de données de validation

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  WB analysis using 86487-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86487-1-RR (WRAP53 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86487-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86487-1-RR against Human WRAP53 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules A431, cellules HEK-293, cellules HeLa, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86487-1-RR cible WRAP53 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: WRAP53 Protéine recombinante Ag6522
• Nom complet: WD repeat containing, antisense to TP53
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 72-75 kDa
• Numéro d’acquisition GenBank: BC002336
• Symbole du gène: WRAP53
• Identification du gène (NCBI): 55135
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WRAP53 (WD40-encoding RNA Antisense to p53), also known as TCAB1 and WDR79, is a multifunctional protein encoded by a gene that produces both a coding mRNA and a regulatory antisense RNA targeting the tumor suppressor p53. Initially identified for its role in maintaining genomic stability by regulating p53 expression, WRAP53 is crucial for telomerase assembly and localization to Cajal bodies, impacting telomere maintenance. It also participates in DNA damage repair and cellular stress responses. The calculated molecular weight of WRAP53 is about 59 kDa, but the actual observed molecular weight is about 72-75 kDa, which may be due to modification.

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