Anticorps Polyclonal de lapin anti-WSB1

WSB1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11666-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 11666-1-AP (same clone as 11666-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11666-1-AP (WSB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11666-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11666-1-AP (same clone as 11666-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11666-1-AP (WSB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11666-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11666-1-AP (same clone as 11666-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WSB1 antibody (11666-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11666-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11666-1-PBS cible WSB1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WSB1 Protéine recombinante Ag2211
• Nom complet: WD repeat and SOCS box-containing 1
• Masse moléculaire calculée: 421 aa, 47 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC021110
• Symbole du gène: WSB1
• Identification du gène (NCBI): 26118
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

WSB1 (or SWIP1) gene encodes WD repeat and SOCS box-containing protein 1, a member of the WD-protein subfamily. It contains several WD-repeats spanning most of the protein and an SOCS box in the C-terminus. Alternatively spliced transcript variants encoding 3 distinct isoforms have been found for this gene. WSB1 serves as a probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Ubiquitination and degradation of homeodomain-interacting protein kinase 2 by WSB1 has been reported (PMID: 15965468), initiated by the interaction of the two components (PMID: 18093972). WSB1 is also a part of an E3 ubiquitin ligase for the thyroid-hormone-activating type 2 iodothyronine deiodinase (D2) (PMID: 15965468).

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