Anticorps Polyclonal de lapin anti-WSB2

• Phare
• Validé par KD/KO

WSB2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12124-2-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of PC-3 using 12124-2-AP (same clone as 12124-2-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12124-2-AP (same clone as 12124-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 12124-2-AP (same clone as 12124-2-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 12124-2-AP (same clone as 12124-2-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 12124-2-AP (same clone as 12124-2-PBS)

  IP result of anti-WSB2 (IP:12124-2-AP, 3ug; Detection:12124-2-AP 1:800) with mouse kidney tissue lysate 6000ug. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 12124-2-AP (same clone as 12124-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using WSB2 antibody (12124-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

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Informations sur le produit

12124-2-PBS cible WSB2 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WSB2 Protéine recombinante Ag2768
• Nom complet: WD repeat and SOCS box-containing 2
• Masse moléculaire calculée: 404 aa, 45 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC015887
• Symbole du gène: WSB2
• Identification du gène (NCBI): 55884
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

WSB2, also named SBA2, encodes WD repeat and SOCS box-containing protein 2. WSB2 may be a substrate-recognition component of an E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. WSB2 is a novel protein supposed to be involved in male- and female-specific gonadal development, and also a regulator of IL-21 receptor expression and IL21-induced signal transduction. WSB2 might be a promising novel biomarker of cell differentiation in colorectal cancer and its biological functions need further studies.

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