Anticorps Recombinant de lapin anti-WT1

WT1 Recombinant Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2J13

N° de cat : 82525-1-PBS

Synonymes

2J13, Wilms tumor 1, Wilms tumor protein

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Galerie de données de validation

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  WB analysis using 82525-1-RR (same clone as 82525-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82525-1-RR (WT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82525-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 82525-1-RR (same clone as 82525-1-PBS)

  IP result of anti-WT1 (IP:82525-1-RR, 4ug; Detection:82525-1-RR 1:1000) with K-562 cells lysate 1600 ug. This data was developed using the same antibody clone with 82525-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82525-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82525-1-RR against Human WT1 were performed. The affinity constant is 0.912 nM.

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Informations sur le produit

82525-1-PBS cible WT1 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: WT1 Protéine recombinante Ag19541
• Nom complet: Wilms tumor 1
• Masse moléculaire calculée: 449 aa, 49 kDa, 57 kDa
• Poids moléculaire observé: 52-55 kDa
• Numéro d’acquisition GenBank: BC032861
• Symbole du gène: WT1
• Identification du gène (NCBI): 7490
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for the normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. The function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has a lower affinity for DNA and can bind RNA. WT1 exists some isoforms with a range of molecular weight is 33-50 kDa.

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