Anticorps Monoclonal anti-WTAP

• Phare
• Validé par KD/KO

WTAP Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

65

CloneNo.

4A10G9

Avis (2)

N° de cat : 60188-1-Ig

Synonymes

Female lethal(2)D homolog, hFL(2)D, KIAA0105, WT1 associated protein, WTAP

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Galerie de données de validation

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  WB analysis using 60188-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60188-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IHC staining of human colon using 60188-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60188-1-Ig (WTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60188-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60188-1-Ig (WTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 60188-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 60188-1-Ig(WTAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules LNCaP, cellules NIH/3T3
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 55 publications below
• IHC: See 11 publications below
• IF: See 6 publications below
• IP: See 4 publications below
• FC: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

60188-1-Ig cible WTAP dans les applications de WB, RIP, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Drosophile, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: WTAP Protéine recombinante Ag0259
• Nom complet: Wilms tumor 1 associated protein
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC000383
• Symbole du gène: WTAP
• Identification du gène (NCBI): 9589
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.

Publications

Species	Application	Title
mouse	WB	Science; Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.; Authors - Shay Geula
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
mouse	IHC, IF	Nat Neurosci; Synaptic N6-methyladenosine (m6A) epitranscriptome reveals functional partitioning of localized transcripts.; Authors - Daria Merkurjev
human	WB,IP	Mol Cell; Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m 6 A Methylation; Authors - Hui-Lung Sun
human	WB	Mol Cell; Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.; Authors - Nandan S Gokhale
human	WB	Mol Cell; mTORC1 promotes cell growth via m6A-dependent mRNA degradation.; Authors - Sungyun Cho ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elaine (Verified Customer) (01-20-2021)

Antibody works fine in Western blot on MEF cells

• Applications: Western Blot

FH

Daniel (Verified Customer) (06-25-2019)

RCC4 grown in normoxic (left lane) and hypoxic (right lane) conditions.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RCC4 human cells

WTAP Antibody Western Blot, validation (1:2000 dilution) in RCC4 human cells (Cat no:60188-1-Ig)

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