Anticorps Monoclonal anti-WTAP

• Phare
• Validé par KD/KO

WTAP Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4A10G9

N° de cat : 60188-1-PBS

Synonymes

4A10G9, Female-lethal(2)D homolog (hFL(2)D ), FL2D, KIAA0105, Mum2

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Galerie de données de validation

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  WB analysis using 60188-1-Ig (same clone as 60188-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  WB analysis using 60188-1-Ig (same clone as 60188-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60188-1-Ig (same clone as 60188-1-PBS)

  IP result of anti-WTAP (IP:60188-1-Ig, 4ug; Detection:10200-1-AP 1:8000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60188-1-Ig (same clone as 60188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60188-1-Ig (WTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60188-1-Ig (same clone as 60188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60188-1-Ig (WTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60188-1-Ig (same clone as 60188-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 60188-1-Ig(WTAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60188-1-PBS cible WTAP dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: WTAP Protéine recombinante Ag0259
• Nom complet: Wilms tumor 1 associated protein
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC000383
• Symbole du gène: WTAP
• Identification du gène (NCBI): 9589
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.

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