Anticorps Recombinant de lapin anti-WTAP

WTAP Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250657D4

N° de cat : 81987-4-RR

Synonymes

Female-lethal(2)D homolog (hFL(2)D ), FL2D, KIAA0105, Mum2, Pre-mRNA-splicing regulator WTAP

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Galerie de données de validation

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  WB analysis using 81987-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81987-4-RR (WTAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 81987-4-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using WTAP antibody (81987-4-RR, Clone: 250657D4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 81987-4-RR

  Biolayer interferometry (BLl) kinetic assays of 81987-4-RR against Human WTAP were performed. The affinity constant is 5.11 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81987-4-RR cible WTAP dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: WTAP Protéine recombinante Ag0259
• Nom complet: Wilms tumor 1 associated protein
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC000383
• Symbole du gène: WTAP
• Identification du gène (NCBI): 9589
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.

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