Anticorps Polyclonal de lapin anti-WWOX

• Phare
• Validé par KD/KO

WWOX Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 15299-1-AP

Synonymes

D16S432E, FOR, FRA16D, HHCMA56, PRO0128, SDR41C1, WOX1, WWOX

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Galerie de données de validation

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  WB analysis using 15299-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15299-1-AP (WWOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15299-1-AP

  WB result of WWOX antibody (15299-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WWOX transfected MCF-7 cells.

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  WB analysis of mouse skeletal muscle using 15299-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15299-1-AP (WWOX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 15299-1-AP

  IP Result of anti-WWOX (IP:15299-1-AP, 4ug; Detection:15299-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human liver cancer using 15299-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15299-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules MCF-7, tissu de muscle squelettique de souris, tissu hépatique de souris, tissu rénal de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Informations sur le produit

15299-1-AP cible WWOX dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WWOX Protéine recombinante Ag7555
• Nom complet: WW domain containing oxidoreductase
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC003184
• Symbole du gène: WWOX
• Identification du gène (NCBI): 51741
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Hum Mutat; Early infantile onset epileptic encephalopathy 28 due to a homozygous microdeletion involving the WWOX gene in a region of uniparental disomy.; Authors - Mariska Davids
human	WB,IHC	Front Oncol; WWOX-rs13338697 genotype predicts therapeutic efficacy of ADI-PEG 20 for patients with advanced hepatocellular carcinoma; Authors - Yu-De Chu ; KD Validated
human	WB	Molecules; Evodiamine Exerts an Anti-Hepatocellular Carcinoma Activity through a WWOX-Dependent Pathway.; Authors - Che-Yuan Hu
human	WB	Int J Mol Med; WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines.; Authors - Qu Junjie J ; KD Validated
human	IHC	Oncol Lett; Expression and clinical significance of WWOX, Elf5, Snail1 and EMT related factors in epithelial ovarian cancer.; Authors - Yakun Hu
human	WB	Int J Cancer; Remote modulation of WWOX by an intronic variant associated with survival of Chinese gastric cancer patients; Authors - Lei Cheng ; KD Validated