Anticorps Polyclonal de lapin anti-WWOX

• Phare
• Validé par KD/KO

WWOX Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15299-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15299-1-AP (same clone as 15299-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15299-1-AP (WWOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 15299-1-AP (same clone as 15299-1-PBS)

  WB result of WWOX antibody (15299-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WWOX transfected MCF-7 cells. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 15299-1-AP (same clone as 15299-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15299-1-AP (WWOX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 15299-1-AP (same clone as 15299-1-PBS)

  IP result of anti-WWOX (IP:15299-1-AP, 4ug; Detection:15299-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15299-1-AP (same clone as 15299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15299-1-AP (same clone as 15299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15299-1-AP (same clone as 15299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15299-1-AP (same clone as 15299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 15299-1-AP (same clone as 15299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 15299-1-AP (same clone as 15299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15299-1-PBS cible WWOX dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WWOX Protéine recombinante Ag7555
• Nom complet: WW domain containing oxidoreductase
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC003184
• Symbole du gène: WWOX
• Identification du gène (NCBI): 51741
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.

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