Anticorps Polyclonal de lapin anti-Willin

Willin Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21039-1-PBS

Synonymes

FRMD6, EX1, FERM domain containing 6, FERM domain-containing protein 6

---

Galerie de données de validation

• 

  WB analysis using 21039-1-AP (same clone as 21039-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21039-1-AP (Willin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21039-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 21039-1-AP (same clone as 21039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21039-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 21039-1-AP (same clone as 21039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21039-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 21039-1-AP (same clone as 21039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21039-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 21039-1-AP (same clone as 21039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21039-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 21039-1-AP (same clone as 21039-1-PBS)

  1X10^6 A431 cells were stained with .2ug Willin antibody (21039-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500. This data was developed using the same antibody clone with 21039-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

21039-1-PBS cible Willin dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Willin Protéine recombinante Ag15375
• Nom complet: FERM domain containing 6
• Masse moléculaire calculée: 622 aa, 72 kDa
• Poids moléculaire observé: 72, 70, 30 kDa
• Numéro d’acquisition GenBank: BC029870
• Symbole du gène: FRMD6/Willin
• Identification du gène (NCBI): 122786
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Willin, also known as FRMD6 or human Expanded (hEx), is a member of the FERM-domain family of cytoskeletal crosslinkers. It is a potent inhibitor of cell proliferation, transformation and modulator of drug sensitivity, suggesting it possesses tumor suppressor properties. Willin is widely expressed in normal tissues. Although willin is found in the cytoplasm and membrane, it localizes to the nuclei of almost all tumors.This antibody was raised against the C-terminal region of human FRMD6 protein and is expected to recognize the endogenous FRMD6.

{{ptg:RelatedPrimaryAntibodies}}