Anticorps Polyclonal de lapin anti-XAB2

• Phare
• Validé par KD/KO

XAB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (3)

N° de cat : 10637-1-AP

Synonymes

Pre-mRNA-splicing factor SYF1, PP3898, NTC90, KIAA1177, HCRN

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Galerie de données de validation

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  WB analysis using 10637-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10637-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10637-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10637-1-AP

  IP result of anti-XAB2 (IP:10637-1-AP, 4ug; Detection:10637-1-AP 1:1000) with K-562 cells lysate 1600 ug.

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  IHC staining of mouse salivary gland using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse salivary gland using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat salivary gland using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat salivary gland using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 10637-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using XAB2 antibody (10637-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562, cellules Y79
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: rat salivary gland tissue, tissu testiculaire de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

10637-1-AP cible XAB2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: XAB2 Protéine recombinante Ag0999
• Nom complet: XPA binding protein 2
• Masse moléculaire calculée: 100 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC007208
• Symbole du gène: XAB2
• Identification du gène (NCBI): 56949
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

XPA-binding protein 2 (XAB2) a tetratricopeptide repeat protein involved in transcription-coupled DNA repair and transcription. XAB2 is involved in pre-mRNA splicing, transcription, and transcription-coupled DNA repair, leading to preimplantation lethality, and is essential for mouse embryogenesis. It interactes with Cockayne syndrome groups A and B (CSA and CSB) proteins and RNA polymerase II, which are known to be involved in transcription-coupled repair (TCR) and transcription, and that the antibodies against XAB2 protein inhibited the recovery of RNA synthesis after UV irradiation and normal RNA synthesis when microinjected into living fibroblasts.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou ; KD Validated
human	WB	Nucleic Acids Res; Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export.; Authors - Lei Haixin H ; KD Validated
human	WB,IF	Cell Death Dis; XAB2 functions in mitotic cell cycle progression via transcriptional regulation of CENPE.; Authors - Shuai Hou ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-19-2025)

Antibody recognizes a specific band of Xab2 in human RKO cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: RKO

XAB2 Antibody Western Blot validation (1:2500 dilution) in RKO (Cat no:10637-1-AP)

FH

Moritz (Verified Customer) (08-19-2024)

Antibody worked well for WB, showing a band at ~ 100kDa using HEK293T WT cell lysate, a dilution of 1:3000 and 1h incubation time at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T WT lysate

FH

Tsimafei (Verified Customer) (08-12-2024)

Antibody detect a strong band ~ 100 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

XAB2 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:10637-1-AP)

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