Anticorps Polyclonal de lapin anti-XAB2

• Phare
• Validé par KD/KO

XAB2 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10637-1-AP

Synonymes

HCNP, HCRN, KIAA1177, NTC90, Pre mRNA splicing factor SYF1, Protein HCNP, SYF1, XAB2, XPA binding protein 2

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Galerie de données de validation

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  WB analysis of K-562 using 10637-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10637-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10637-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10637-1-AP

  IP Result of anti-XAB2 (IP:10637-1-AP, 4ug; Detection:10637-1-AP 1:200) with K-562 cells lysate 3200ug.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa
• Résultats positifs en IP: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

10637-1-AP cible XAB2 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: XAB2 Protéine recombinante Ag0999
• Nom complet: XPA binding protein 2
• Masse moléculaire calculée: 100 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC007208
• Symbole du gène: XAB2
• Identification du gène (NCBI): 56949
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

XPA-binding protein 2 (XAB2) a tetratricopeptide repeat protein involved in transcription-coupled DNA repair and transcription. XAB2 is involved in pre-mRNA splicing, transcription, and transcription-coupled DNA repair, leading to preimplantation lethality, and is essential for mouse embryogenesis. It interactes with Cockayne syndrome groups A and B (CSA and CSB) proteins and RNA polymerase II, which are known to be involved in transcription-coupled repair (TCR) and transcription, and that the antibodies against XAB2 protein inhibited the recovery of RNA synthesis after UV irradiation and normal RNA synthesis when microinjected into living fibroblasts.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou ; KD Validated
human	WB	Nucleic Acids Res; Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export.; Authors - Lei Haixin H ; KD Validated
human	WB,IF	Cell Death Dis; XAB2 functions in mitotic cell cycle progression via transcriptional regulation of CENPE.; Authors - Shuai Hou ; KD Validated