Anticorps Polyclonal de lapin anti-XAF1

XAF1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 27057-1-AP

Synonymes

BIRC4 binding protein, BIRC4-binding protein, BIRC4BP, XIAP associated factor 1, XIAPAF1

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Galerie de données de validation

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  WB analysis using 27057-1-AP

  Untreated and IFN alpha treated treated Jurkat cells were subjected to SDS PAGE followed by western blot with 27057-1-AP (XAF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 27057-1-AP

  Untreated and IFN alpha treated treated Jurkat cells were subjected to SDS PAGE followed by western blot with 27057-1-AP (XAF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 27057-1-AP

  IP result of anti-XAF1 (IP:27057-1-AP, 4ug; Detection:27057-1-AP 1:800) with Jurkat cells lysate 1920 ug.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, IFN alpha treated treated Jurkat cells
• Résultats positifs en IP: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27057-1-AP cible XAF1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: XAF1 Protéine recombinante Ag25843
• Nom complet: XIAP associated factor 1
• Masse moléculaire calculée: 301 aa, 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC073156
• Symbole du gène: XAF1
• Identification du gène (NCBI): 54739
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

XIAP-associated factor 1 (XAF1) is also named as BIRC4BP (BIRC4-binding protein) and XIAPAF1. XAF1 was originally identified as an antagonist of XIAP counteracting its anti-caspase activity by inhibiting the RING domain of XIAP. XAF1is also concerned with IFN-induced apoptosis (PMID: 35972291). XAF1 plays a key role in the communication between IRF1-mediated early immune responses and IRF3-mediated late IFN-dependent immune responses (PMID: 37595039). XAF1 as a direct interacting protein of AKT1, which strongly binds the N-terminal region of AKT1 to block its K63-linked poly-ubiquitination and subsequent activation (PMID: 37384528). XAF1 may serve as an osteoprotective regulator to restore bone homeostasis (PMID: 38957269).

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