Anticorps Recombinant de lapin anti-XBP1S-specific

XBP1S-specific Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241004B6

N° de cat : 83959-5-PBS

Synonymes

XBP1, 241004B6, Tax-responsive element-binding protein 5, TREB-5, X box binding protein 1

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Galerie de données de validation

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  WB analysis using 83959-5-RR (same clone as 83959-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83959-5-RR (XBP1S-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83959-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 83959-5-RR (same clone as 83959-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83959-5-RR (XBP1S-specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83959-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 83959-5-RR (same clone as 83959-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83959-5-RR (XBP1S-specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83959-5-PBS in a different storage buffer formulation.

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  FC experiment of Ramos using 83959-5-RR (same clone as 83959-5-PBS)

  1x10^6 Ramos cells were intracellularly stained with 0.25 ug XBP1S-specific Recombinant antibody (83959-5-RR, Clone:241004B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83959-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83959-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83959-5-RR against Human XBP1S-specific were performed. The affinity constant is 1.08 nM.

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Informations sur le produit

83959-5-PBS cible XBP1S-specific dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: XBP1S-specific Protéine recombinante Ag21703
• Nom complet: X-box binding protein 1
• Masse moléculaire calculée: 261 aa, 29 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC000938
• Symbole du gène: XBP1
• Identification du gène (NCBI): 7494
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

XBP1, also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). XBP1 localizes in the nucleus. As a transcription factor, it is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The calculated molecular weight of protein generated from the unspliced XBP1 isoform XBP1U mRNA is 33 kDa, and protein generated from spliced XBP1 isoform XBP1S mRNA is 54 kDa. This antibody specially recognizes the XBP1S isoform.

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