Anticorps Polyclonal de lapin anti-XDH

• Phare
• Validé par KD/KO

XDH Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(26)

N° de cat : 55156-1-AP

Synonymes

XO, XDHA, XD, Xanthine oxidoreductase, Xanthine Oxidase

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Galerie de données de validation

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  WB analysis of mouse liver using 55156-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55156-1-AP (XDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 55156-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 55156-1-AP (XDH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55156-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55156-1-AP (XDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55156-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55156-1-AP (XDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 55156-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55156-1-AP (XDH antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 55156-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55156-1-AP (XDH antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 55156-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55156-1-AP (XDH antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55156-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55156-1-AP (XDH antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu cardiaque humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 25 publications below
• IF: See 1 publications below

Informations sur le produit

55156-1-AP cible XDH dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, poisson-zèbre, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: xanthine dehydrogenase
• Masse moléculaire calculée: 146 kDa
• Poids moléculaire observé: 147-150 kDa
• Numéro d’acquisition GenBank: NM_000379
• Symbole du gène: XDH
• Identification du gène (NCBI): 7498
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

XDH, also named as XDHA, XO and XD, belongs to the xanthine dehydrogenase family. It catalyzes the last two steps of purine catabolism in man, formation of the end product uric acid from hypoxanthine and xanthine. The mammalian enzyme is synthesized as a dehydrogenase (XDH), which uses NAD as the electron acceptor, but it can be converted into an oxidase (XO) both in vivo and in vitro.This gene encodes a protein of 150 kDa and the 150 kDa polypeptide can be processed into a fragment of 130 kDa and further to 85 kDa by spontaneous proteolytic cleavage(PMID:9989587). In addition, the full length polypeptide can be also partially processed into a 87 kDa and 59 kDa fragments(PMID:21528298). It also can exsit as a homodimer(PMID:9989587). This antibody is specific to XDH.

Publications

Species	Application	Title
mouse,human	WB	Int J Mol Sci; Multiple-Purpose Connectivity Map Analysis Reveals the Benefits of Esculetin to Hyperuricemia and Renal Fibrosis.; Authors - Yiming Wang
mouse	WB	Free Radic Biol Med; PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.; Authors - Xue Chen
mouse	WB	Nutrients; Chinese Sumac (Rhus chinensis Mill.) Fruits Prevent Hyperuricemia and Uric Acid Nephropathy in Mice Fed a High-Purine Yeast Diet; Authors - Nan Ma
mouse	WB	Biochem Pharmacol; PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1.; Authors - Yunhui Xu
mouse	WB	Sci Rep; Iron regulatory protein deficiency compromises mitochondrial function in murine embryonic fibroblasts.; Authors - Huihui Li
chicken	WB	Poult Sci; Effects of Smilax China L. extracts on Hyperuricemia chicken model via inhibiting xanthine oxidase activity; Authors - Mingen Yan

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