Anticorps Polyclonal de lapin anti-XIAP

• Phare
• Validé par KD/KO

XIAP Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(51)

N° de cat : 10037-1-Ig

Synonymes

API3, Baculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, EC:2.3.2.27, hIAP-3

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Galerie de données de validation

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  WB analysis using 10037-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10037-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10037-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 10037-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10037-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10037-1-Ig(XIAP antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules C6, cellules COLO 320, cellules HeLa, cellules HepG2, cellules Jurkat, cellules NIH/3T3, cellules Raji
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 45 publications below
• IHC: See 11 publications below
• CoIP: See 1 publications below

Informations sur le produit

10037-1-Ig cible XIAP dans les applications de WB, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: XIAP Protéine recombinante Ag0022
• Nom complet: X-linked inhibitor of apoptosis
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 45-57 kDa
• Numéro d’acquisition GenBank: BC032729
• Symbole du gène: XIAP
• Identification du gène (NCBI): 331
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer.; Authors - Kai Yuan
human	WB	Cancer Res; DAB2IP is a bifunctional tumor suppressor that regulates wildtype RAS and inflammatory cascades in KRAS mutant colon cancer; Authors - Abigail L Miller
human	IHC	Cancer Res; An ATM/TRIM37/NEMO axis counteracts genotoxicity by activating nuclear-to-cytoplasmic NF-κB signaling.; Authors - Geyan Wu
human	WB	EMBO Rep; XIAP-mediated degradation of IFT88 disrupts HSC cilia to stimulate HSC activation and liver fibrosis; Authors - Renjie Hong ; KD Validated
human	WB	Acta Pharmacol Sin; Aspirin modulates succinylation of PGAM1K99 to restrict the glycolysis through NF-κB/HAT1/PGAM1 signaling in liver cancer.; Authors - Yu-Fei Wang
human	WB	Int J Nanomedicine; Synergism of cisplatin-oleanolic acid co-loaded calcium carbonate nanoparticles on hepatocellular carcinoma cells for enhanced apoptosis and reduced hepatotoxicity.; Authors - Muhammad Waseem Khan

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