Anticorps Polyclonal de lapin anti-XIAP

XIAP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 23453-1-AP

Synonymes

IAP-3, hIAP-3, EC:2.3.2.27, E3 ubiquitin-protein ligase XIAP, Baculoviral IAP repeat-containing protein 4

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Galerie de données de validation

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  WB analysis using 23453-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23453-1-AP (XIAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23453-1-AP

  Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 23453-1-AP (XIAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 23453-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 23453-1-AP (XIAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23453-1-AP (XIAP antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 23453-1-AP (XIAP antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 23453-1-AP (XIAP antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23453-1-AP (XIAP antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23453-1-AP (XIAP antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 23453-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 23453-1-AP (XIAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HEK-293, cellules HepG2 traitées à la tunicamycine, cellules NIH/3T3
• Résultats positifs en IHC: tissu de côlon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

23453-1-AP cible XIAP dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: XIAP Protéine recombinante Ag0725
• Nom complet: X-linked inhibitor of apoptosis
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC032729
• Symbole du gène: XIAP
• Identification du gène (NCBI): 331
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56kd.

Publications

Species	Application	Title
human	WB,IHC	Cancer Lett; Esculetin inhibits endometrial cancer proliferation and promotes apoptosis via hnRNPA1 to downregulate BCLXL and XIAP.; Authors - Ruqi Jiang
human	WB,IF	Biochim Biophys Acta Mol Cell Res; HTRA3 transcriptionally inhibited by FOXP1 suppresses tumorigenesis of osteosarcoma via the PTEN/PI3K/AKT pathway; Authors - Ziyuan Tong
human	WB	Front Immunol; Metabolic Reprogramming Mediates Delayed Apoptosis of Human Neutrophils Infected With Francisella tularensis.; Authors - Samantha J Krysa
human	WB	Environ Pollut; DBDPE upregulates NOD-like receptor signaling to induce NLRP3 inflammasome-mediated HAECs pyroptosis; Authors - Li Jing

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