Anticorps Monoclonal anti-XLF

XLF Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F8C7

N° de cat : 66552-1-PBS

Synonymes

NHEJ1, Protein cernunnos, XLF, XRCC4 like factor

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Galerie de données de validation

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  WB analysis using 66552-1-Ig (same clone as 66552-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66552-1-Ig (XLF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66552-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66552-1-Ig (same clone as 66552-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66552-1-Ig (XLF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66552-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66552-1-Ig (same clone as 66552-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66552-1-Ig (XLF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66552-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66552-1-Ig (same clone as 66552-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66552-1-Ig (NHEJ1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66552-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66552-1-Ig (same clone as 66552-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66552-1-Ig (NHEJ1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66552-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66552-1-PBS cible XLF dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: XLF Protéine recombinante Ag2496
• Nom complet: nonhomologous end-joining factor 1
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC030986
• Symbole du gène: XLF
• Identification du gène (NCBI): 79840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.

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