Anticorps Polyclonal de lapin anti-XRCC1

• Phare
• Validé par KD/KO

XRCC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 21468-1-AP

Synonymes

DNA repair protein XRCC1, X-ray repair cross-complementing protein 1

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Galerie de données de validation

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  WB analysis using 21468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 21468-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 21468-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21468-1-AP (XRCC1 antibody) at dilution of 1:1000 (under 40x lens).

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  IF Staining of A431 using 21468-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using XRCC1 antibody (21468-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 1 publications below

Informations sur le produit

21468-1-AP cible XRCC1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: XRCC1 Protéine recombinante Ag15172
• Nom complet: X-ray repair complementing defective repair in Chinese hamster cells 1
• Masse moléculaire calculée: 633 aa, 70 kDa
• Poids moléculaire observé: 80-90 kDa
• Numéro d’acquisition GenBank: BC023593
• Symbole du gène: XRCC1
• Identification du gène (NCBI): 7515
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

XRCC1 (X-ray Repair Cross Complementing group 1) is a molecular scaffold protein that interacts with multiple enzymatic components of DNA single-strand break repair (SSBR) including DNA kinase, DNA phosphatase, DNA polymerase, DNA deadenylase, and DNA ligase activities that collectively are capable of accelerating the repair of a broad range of DNA single-strand breaks (SSBs) (PMID: 18515191). XRCC1 is 633 amino acids in length which is comprised of an N-terminal domain (NTD) of ˜160 amino acids that interacts with Pol β, a central BRCT domain of ˜90 amino acids that interacts with PARP1 (PMID: 9584196). This antibody is not suitable for murine samples.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response.; Authors - Mingming Yang
human	WB	Mol Ther; PMID: 29456019; Authors - Xinyi Wang
human	WB	J Cell Mol Med; Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs.; Authors - Xingjie Gao
human	WB	Int J Environ Res Public Health; Effects of DNA Damage and Oxidative Stress in Human Bronchial Epithelial Cells Exposed to PM2.5 from Beijing, China, in Winter.; Authors - Bing-Yu Niu
human	WB	Toxicol Appl Pharmacol; Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.; Authors - Hui Dong
human	WB,IHC	Front Mol Biosci; XRCC1 Is a Promising Predictive Biomarker and Facilitates Chemo-Resistance in Gallbladder Cancer.; Authors - Zhengchun Wu ; KD Validated

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