Anticorps Monoclonal anti-XRCC2

XRCC2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

3D9A1

N° de cat : 66652-1-PBS

Synonymes

3D9A1, DNA repair protein XRCC2, X-ray repair cross-complementing protein 2

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Galerie de données de validation

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  WB analysis of HEK-293 using 66652-1-Ig (same clone as 66652-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66652-1-Ig (XRCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66652-1-Ig (same clone as 66652-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66652-1-Ig (XRCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66652-1-Ig (same clone as 66652-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66652-1-Ig (XRCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66652-1-Ig (same clone as 66652-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC2 antibody (66652-1-Ig, Clone: 3D9A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66652-1-Ig (same clone as 66652-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC2 antibody (66652-1-Ig, Clone: 3D9A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66652-1-Ig (same clone as 66652-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XRCC2 (66652-1-Ig, Clone:3D9A1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66652-1-PBS cible XRCC2 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: XRCC2 Protéine recombinante Ag14421
• Nom complet: X-ray repair complementing defective repair in Chinese hamster cells 2
• Masse moléculaire calculée: 280 aa, 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC042137
• Symbole du gène: XRCC2
• Identification du gène (NCBI): 7516
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway.

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