Anticorps Polyclonal de lapin anti-XRCC4

• Phare
• Validé par KD/KO

XRCC4 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(15)

Avis (1)

N° de cat : 15817-1-AP

Synonymes

X-ray repair cross-complementing protein 4, Protein XRCC4, C-terminus, hXRCC4, DNA repair protein XRCC4

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Galerie de données de validation

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  WB analysis of HepG2 using 15817-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15817-1-AP (XRCC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15817-1-AP

  WB result of XRCC4 antibody (15817-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XRCC4 transfected HEK-293 cells.

• 

  WB analysis of COLO 320 using 15817-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15817-1-AP (XRCC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15817-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15817-1-AP (XRCC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15817-1-AP

  IP result of anti-XRCC4 (IP:15817-1-AP, 4ug; Detection:15817-1-AP 1:800) with HepG2 cells lysate 3200ug.

• 

  IHC staining of human breast cancer using 15817-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15817-1-AP (XRCC4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15817-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15817-1-AP (XRCC4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 15817-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine using 15817-1-AP (XRCC4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of rat small intestine using 15817-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine using 15817-1-AP (XRCC4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of rat testis using 15817-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 15817-1-AP (XRCC4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of rat testis using 15817-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 15817-1-AP (XRCC4 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules COLO 320, cellules HEK-293
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu d'intestin grêle de rat, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

15817-1-AP cible XRCC4 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: XRCC4 Protéine recombinante Ag8606
• Nom complet: X-ray repair complementing defective repair in Chinese hamster cells 4
• Masse moléculaire calculée: 310 aa, 35 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC005259
• Symbole du gène: XRCC4
• Identification du gène (NCBI): 7518
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). XRCC4 is one of the core machiney of NHEJ that required for double-strand break repair and V(D)J recombination. The DNA ligase IV(LIG4)-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4.

Publications

Species	Application	Title
human	WB	Nat Commun; Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.; Authors - Guijie Guo ; KD Validated
human	WB	Cancer Res; Inhibition of ATM Induces Hypersensitivity to Proton Irradiation by Upregulating Toxic End Joining.; Authors - Qin Zhou
		PLoS Biol; A long noncoding RNA sensitizes genotoxic treatment by attenuating ATM activation and homologous recombination repair in cancers.; Authors - Kunming Zhao
human	WB	Cell Death Differ; LRIK interacts with the Ku70-Ku80 heterodimer enhancing the efficiency of NHEJ repair.; Authors - Dan Wang
human	WB	Phytomedicine; Guiqi Baizhu decoction enhances radiosensitivity in non-small cell lung cancer by inhibiting the HIF-1α/DNA-PKcs axis-mediated DNA repair; Authors - Yangyang Li
human	WB	Toxicol Appl Pharmacol; Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.; Authors - Hui Dong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alexandra (Verified Customer) (07-02-2024)

For plasmid expression it works fine with 1000x dilution, but for the detection of endogenous XRCC4 I recommend the use of 500x dilution.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation, Cell culture
• Primary Antibody Dilution: 1000x
• Cell Tissue Type: U2OS

XRCC4 Antibody Western Blot,Immunofluorescence,Immunoprecipitation,Cell culture validation (1000x dilution) in U2OS (Cat no:15817-1-AP)

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