Anticorps Monoclonal anti-XRCC5/Ku80

XRCC5/Ku80 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G5E7

N° de cat : 66546-1-PBS

Synonymes

KU80, XRCC5, 2G5E7, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit

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Galerie de données de validation

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  WB analysis using 66546-1-Ig (same clone as 66546-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66546-1-Ig (XRCC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

  IP result of anti-XRCC5/Ku80 (IP:66546-1-Ig, 5ug; Detection:66546-1-Ig 1:20000) with HeLa cells lysate 3200 ug. This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

  IP result of anti-XRCC5/Ku80 (IP:66546-1-Ig, 5ug; Detection:66546-1-Ig 1:40000) with HeLa cells lysate 640 ug. This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66546-1-Ig (same clone as 66546-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66546-1-Ig (XRCC5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66546-1-Ig (same clone as 66546-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66546-1-Ig (XRCC5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66546-1-Ig (same clone as 66546-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66546-1-Ig (XRCC5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66546-1-Ig (same clone as 66546-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66546-1-Ig (XRCC5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using XRCC5 antibody (66546-1-Ig, Clone: 2G5E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66546-1-Ig (XRCC5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66546-1-PBS cible XRCC5/Ku80 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: XRCC5/Ku80 Protéine recombinante Ag9512
• Nom complet: X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
• Masse moléculaire calculée: 732 aa, 83 kDa
• Poids moléculaire observé: 80-83 kDa
• Numéro d’acquisition GenBank: BC019027
• Symbole du gène: XRCC5
• Identification du gène (NCBI): 7520
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.

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