Anticorps Recombinant de lapin anti-XRCC5/Ku80

XRCC5/Ku80 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240301G6

N° de cat : 80325-6-PBS

Synonymes

KU80, XRCC5, 240301G6, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit

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Galerie de données de validation

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  WB analysis using 80325-6-RR (same clone as 80325-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80325-6-RR (XRCC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 80325-6-RR (same clone as 80325-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80325-6-RR (XRCC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 80325-6-RR (same clone as 80325-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80325-6-RR (XRCC5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80325-6-RR (same clone as 80325-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC5 antibody (80325-6-RR, Clone: 240301G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 80325-6-RR (same clone as 80325-6-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug XRCC5 Recombinant antibody (80325-6-RR, Clone:240301G6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80325-6-RR

  Biolayer interferometry (BLl) kinetic assays of 80325-6-RR against Human XRCC5 were performed. The affinity constant is 4.59 nM.

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Informations sur le produit

80325-6-PBS cible XRCC5/Ku80 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: XRCC5/Ku80 Protéine recombinante Ag9454
• Nom complet: X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
• Masse moléculaire calculée: 732 aa, 83 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC019027
• Symbole du gène: XRCC5
• Identification du gène (NCBI): 7520
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication.

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