Anticorps Monoclonal anti-XRCC5

XRCC5 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2G5E7

N° de cat : CL488-66546

Synonymes

86 kDa subunit of Ku antigen, CTC85, CTCBF, DNA repair protein XRCC5, FLJ39089, G22P2, KARP 1, KARP1, KU80, Ku86, KUB2, NFIV, Nuclear factor IV, Thyroid lupus autoantigen, TLAA, XRCC5

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Galerie de données de validation

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  IF Staining of HepG2 using CL488-66546

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66546 (XRCC5 antibody) at dilution of 1:100.

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  IF Staining of HeLa using CL488-66546

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL488-66546 (XRCC5 antibody) at dilution of 1:100.

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  IF Staining of HeLa using CL488-66546

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488-conjugated XRCC5 antibody (CL488-66546, Clone: 2G5E7 ) at dilution of 1:100.

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  FC experiment of HeLa using CL488-66546

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human XRCC5 (CL488-66546, Clone:2G5E7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF: cellules HepG2, cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66546 cible XRCC5 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: XRCC5 Protéine recombinante Ag9512
• Nom complet: X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
• Masse moléculaire calculée: 732 aa, 83 kDa
• Poids moléculaire observé: 80-83 kDa
• Numéro d’acquisition GenBank: BC019027
• Symbole du gène: XRCC5
• Identification du gène (NCBI): 7520
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011