Anticorps Polyclonal de lapin anti-XRN2

• Phare
• Validé par KD/KO

XRN2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 11267-1-AP

Synonymes

5 3 exoribonuclease 2, 5' 3' exoribonuclease 2, DHM1 like protein, DHP protein, XRN2

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Galerie de données de validation

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  WB analysis using 11267-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 11267-1-AP (XRN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11267-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11267-1-AP (XRN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11267-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11267-1-AP (XRN2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11267-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11267-1-AP (XRN2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules COLO 320, cellules HEK-293, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

11267-1-AP cible XRN2 dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: XRN2 Protéine recombinante Ag1788
• Nom complet: 5'-3' exoribonuclease 2
• Masse moléculaire calculée: 104 kDa
• Poids moléculaire observé: 104 kDa
• Numéro d’acquisition GenBank: BC006417
• Symbole du gène: XRN2
• Identification du gène (NCBI): 22803
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	Cell; Microprocessor, Setx, Xrn2, and Rrp6 co-operate to induce premature termination of transcription by RNAPII.; Authors - Wagschal Alexandre A ; KD Validated
human	ChIP,CoIP,WB	EMBO J; Arginine methylation of the DDX5 helicase RGG/RG motif by PRMT5 regulates resolution of RNA:DNA hybrids.; Authors - Sofiane Y Mersaoui ; KD Validated
		J Cell Biol; Intronless mRNAs transit through nuclear speckles to gain export competence.; Authors - Ke Wang
human	WB	PLoS Pathog; Characterization of miR-122-independent propagation of HCV.; Authors - Chikako Ono ; KD Validated
human	WB, IF	J Virol; Dissecting the roles of the 5' exoribonucleases xrn1 and xrn2 in restricting hepatitis C virus replication.; Authors - You Li ; KD Validated