Anticorps Polyclonal de lapin anti-XYLT1

XYLT1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 55061-1-AP

Synonymes

Peptide O xylosyltransferase 1, XT I, XT1, Xylosyltransferase 1, xylosyltransferase I, XylT I, XYLT1

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Galerie de données de validation

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  WB analysis of HEK-293 using 55061-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55061-1-AP (XYLT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 55061-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55061-1-AP (XYLT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 55061-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 55061-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 55061-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse pancreas using 55061-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules BxPC-3
• Résultats positifs en IHC: tissu rénal de souris, tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

55061-1-AP cible XYLT1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: xylosyltransferase I
• Masse moléculaire calculée: 108 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: NM_022166
• Symbole du gène: XYLT1
• Identification du gène (NCBI): 64131
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

XYLT1, also named as XT1, Belongs to the glycosyltransferase 14 family and XylT subfamily. It catalyzes the first step in biosynthesis of glycosaminoglycan. XYLT1 transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes. The antibody is specific to XYLT1.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Gua Lou Er Chen decoction attenuates atherosclerosis by reducing proteoglycans accumulation and inflammation; Authors - Hongya Guo
human	WB	J Biol Chem; Chondroitin sulfate modification of CSPG4 regulates the maintenance and differentiation of glioma initiating cells via integrin-associated signaling; Authors - Akiko Niibori-Nambu
human	WB,IP,IHC,IF	Cancer Res; The Glycosyltransferase XYLT1 Activates NF-κB Signaling to Promote Metastasis of Early-Stage Lung Adenocarcinoma; Authors - Jian Han

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