- Phare
- Validé par KD/KO
Anticorps Monoclonal anti-YAP1
YAP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugaison
Non conjugué
CloneNo.
3A7A9
N° de cat : 66900-1-Ig
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules NCI-H1299, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules HUVEC, cellules LNCaP, cellules MCF-7, cellules NIH/3T3, cellules NIH-3T3 |
Résultats positifs en IHC | tissu de cancer du poumon humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de tumeur ovarienne humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules HepG2, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochimie (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 12 publications below |
WB | See 65 publications below |
IHC | See 20 publications below |
IF | See 46 publications below |
IP | See 4 publications below |
CoIP | See 7 publications below |
Informations sur le produit
66900-1-Ig cible YAP1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, Humain, souris |
Hôte / Isotype | Mouse / IgG1 |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | YAP1 Protéine recombinante Ag28194 |
Nom complet | Yes-associated protein 1, 65kDa |
Masse moléculaire calculée | 504 aa, 54 kDa |
Poids moléculaire observé | 65-75 kDa |
Numéro d’acquisition GenBank | BC038235 |
Symbole du gène | YAP1 |
Identification du gène (NCBI) | 10413 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine G |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). Phosphorylation of S381 primes YAP phosphorylation by CK1δ/ɛ, resulting in activation of a phosphodegron, thus generates a binding surface that interacts with a ubiquitin ligase, and leads to degradation by ubiquitination. (PMID: 20048001). The calcualted molecular weight of YAP1 is 54 kDa, but routinely observed at 65-75 kDa by Western Blot (PMID: 28230103, 33264286, 36255405).
Protocole
Product Specific Protocols | |
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WB protocol for YAP1 antibody 66900-1-Ig | Download protocol |
IHC protocol for YAP1 antibody 66900-1-Ig | Download protocol |
IF protocol for YAP1 antibody 66900-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cancer Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer | ||
Nat Commun Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation | ||
Nat Commun Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.
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Cancer Lett A novel antagonist of the CCL5/CCR5 axis suppresses the tumor growth and metastasis of triple-negative breast cancer by CCR5-YAP1 regulation
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Acta Pharmacol Sin Adaptor protein CEMIP reduces the chemosensitivity of small cell lung cancer via activation of an SRC-YAP oncogenic module | ||
Oncogene The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Udesh (Verified Customer) (11-26-2023) | Worked well at 1:1000 for WB
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FH Joshua (Verified Customer) (01-29-2019) | Coverslips were fixed with 4%PFA+0.25% Triton-X-100. Primary staining was 1:500, secondary was 1:750 with rockland 488 anti-mouse overnight at 4C.Left panel: Control PANC1 cells.Right panel: PANC1 cells with YAP silencing
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