Anticorps Recombinant de lapin anti-YAP1

YAP1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

5E3

N° de cat : 81090-1-RR

Synonymes

5E3, 65 kDa Yes associated protein, Protein yorkie homolog, Transcriptional coactivator YAP1, YAP

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Galerie de données de validation

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  WB analysis using 81090-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81090-1-RR (YAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 81090-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81090-1-RR (YAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 81090-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 81090-1-RR (YAP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 81090-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 81090-1-RR (YAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 81090-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81090-1-RR (YAP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 81090-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YAP1 antibody (81090-1-RR, Clone: 5E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 81090-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81090-1-RR against Human YAP1 were performed. The affinity constant is 0.716 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules C6, cellules HepG2, cellules HSC-T6, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

81090-1-RR cible YAP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: YAP1 Protéine recombinante Ag4510
• Nom complet: Yes-associated protein 1, 65kDa
• Masse moléculaire calculée: 504 aa, 54 kDa
• Poids moléculaire observé: 65-75 kDa
• Numéro d’acquisition GenBank: BC038235
• Symbole du gène: YAP1
• Identification du gène (NCBI): 10413
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination (20048001). The calcualted molecular weight of YAP1 is 54 kDa, but routinely observed at 65-75 kDa by Western Blot (PMID: 28230103, 33264286, 36255405).

Publications

Species	Application	Title
sheep	IHC	Sci Rep; YAP promotes the early development of temporomandibular joint bony ankylosis by regulating mesenchymal stem cell function; Authors - Tong-Mei Zhang
mouse		Front Immunol; Modified Gegen Qinlian Decoction modulated the gut microbiome and bile acid metabolism and restored the function of goblet cells in a mouse model of ulcerative colitis; Authors - Jinke Huang

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