• Phare
  • Validé par KD/KO

Anticorps Monoclonal anti-YAP1

YAP1 Monoclonal Antibody for IF/ICC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(2)

CloneNo.

3A7A9

N° de cat : CL488-66900

Synonymes

yes associated protein 1, YAP, Transcriptional coactivator YAP1, Protein yorkie homolog, 65 kDa Yes associated protein



Applications testées

Résultats positifs en IF/ICCcellules HepG2,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66900 cible YAP1 dans les applications de WB, IF/ICC et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène YAP1 Protéine recombinante Ag28194
Nom complet Yes-associated protein 1, 65kDa
Masse moléculaire calculée 504 aa, 54 kDa
Numéro d’acquisition GenBankBC038235
Symbole du gène YAP1
Identification du gène (NCBI) 10413
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination (20048001).The calcualted molecular weight of YAP1 is 54 kDa, but routinely observed at 65-75 kDa by Western Blot (PMID: 28230103, 33264286, 36255405).

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 YAP1 antibody CL488-66900Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Glia

Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes

Authors - Yue Wan
humanIF

Biomedicines

Inactivation of Yes-Associated Protein Mediates Trophoblast Dysfunction: A New Mechanism of Pregnancy Loss Associated with Anti-Phospholipid Antibodies?

Authors - Zengshu Huang
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