Anticorps Recombinant de lapin anti-YARS

YARS Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242400E9

N° de cat : 84859-3-PBS

Synonymes

YARS1, 242400E9, CMTDIC, TYRRS, YRS

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Galerie de données de validation

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  WB analysis using 84859-3-RR (same clone as 84859-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84859-3-RR (YARS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84859-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84859-3-RR (same clone as 84859-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84859-3-RR (YARS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84859-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84859-3-RR (same clone as 84859-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84859-3-RR (YARS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84859-3-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 84859-3-RR (same clone as 84859-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using YARS antibody (84859-3-RR, Clone: 242400E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84859-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01634-2

  Cytometric bead array standard curve of MP01634-2, YARS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84859-3-PBS. Detection antibody: 84859-2-PBS. Standard: Ag35148. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 84859-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84859-3-RR against Human YARS were performed. The affinity constant is 0.414 nM.

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Informations sur le produit

84859-3-PBS cible YARS dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: YARS Protéine recombinante Ag35148
• Nom complet: tyrosyl-tRNA synthetase
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC016689
• Symbole du gène: YARS
• Identification du gène (NCBI): 8565
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tyrosyl-tRNA synthetase (YARS) is responsible for tyrosyl-tRNA aminoacylation. The functional enzyme exists as a homodimer, has potent leukocyte and monocyte chemotaxis activity, and stimulates production of myeloperoxidase, tumor necrosis factor-α, and tissue factor. A shift in the monomer:dimer equilibrium could result in dysregulation of the cytokines function. (PMID: 33490854)

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